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Supplementary Figure 2. (A) In situ hybridization examining HDAC1 expression in early embryos from
2-cell to late neurula stages. (B) In situ hybridization examining neural plate border markers Msx2, Ap2,
and Zic1 expression after treatment with vehicle or inhibitor (TSA- 200nM or VPA- 20mM). Embryos
were treated at mid-gastrula stages (stage 11) and collected at mid-neurula stages (stage 15). (C) Explant
assay examining Snail2 and FoxD3 expression in Wnt/Chordin induced explants that were treated with
vehicle or inhibitor (TSA-200nM or VPA-10mM). Explants were cultured alongside sibling embryos
grown until late neurula stages (stage 18). (D) Explant assay examining Snail2 and FoxD3 expression in
Wnt/Chordin induced explants that were treated with vehicle (DMSO) or inhibitor (RMD – 15uM).
Explants were cultured alongside sibling embryos cultured until late neurula stages (stage 18). |
