|
Gene |
Clone |
Species |
Stages |
Anatomy |
hhex.L
|
|
laevis
|
NF stage 9
|
endoderm
,
anterior
|
hhex.L
|
|
laevis
|
NF stage 10
|
endoderm
,
endomesoderm
,
anterior
|
hhex.L
|
|
laevis
|
NF stage 18
|
endoderm
,
liver diverticulum
,
foregut
,
liver primordium
|
hhex.L
|
|
laevis
|
NF stage 25
|
endoderm
,
alimentary system
,
foregut
,
vascular endothelium
,
liver primordium
,
[+]
|
hhex.L
|
|
laevis
|
NF stage 35 and 36
|
alimentary system
,
liver
,
foregut
,
posterior cardinal vein
,
thyroid primordium
,
[+]
|
|
|
Fig. 1. A −6Kb:hhex:gfp transgene recapitulates hhex expression. (A) Diagram of the Xenopus laevis hhex genomic locus and −6.0Kb:hhex:gfp transgene. (B) The transgene recapitulates hhex expression in the anterior endoderm from blastula (st9.5) to stages 25. Transgenic embryos assayed by in situ hybridization for hhex or gfp mRNA and for GFP fluorescence with a confocal (st9.5 and 10) or stereomicroscope (st18–35). Embryos from st9.5 to 18 are bisected; in st9.5–11, dorsal is left; and in st18–35, anterior is right. The GFP image at st35 shows both a transgenic (top) and non-transgenic embryos (bottom). Two independent transgenic lines exhibited identical expression patterns. Arrowheads indicate endothelial cells (black), thyroid (white), and liver bud (yellow). |