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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tbxt
|
|
laevis
|
NF stage 10
|
mesoderm
,
dorsal marginal zone
|
chrd.1
|
|
laevis
|
NF stage 10
|
ectoderm
,
dorsal marginal zone
|
sox17a
|
|
laevis
|
NF stage 10
|
endoderm
,
blastopore
,
vegetal endoderm
|
cer1
|
|
laevis
|
NF stage 10
|
endoderm
,
endomesoderm
|
gsc
|
|
laevis
|
NF stage 10
|
mesoderm
,
dorsal marginal zone
|
myf5
|
|
laevis
|
NF stage 10
|
mesoderm
,
marginal zone
,
dorso-lateral marginal zone
|
foxi1
|
|
laevis
|
NF stage 10
|
ectoderm
,
epidermis
,
animal hemisphere
|
krt12.4
|
|
laevis
|
NF stage 10
|
ectoderm
,
epidermis
,
animal hemisphere
|
|
|
Fig. 2. H3.3/H3-depleted Xenopus embryos fail to express mesodermal marker genes. (A-I′) Control and injected embryos fixed at early gastrula were subjected to RNA in situ hybridization for the analysis of mesodermal (A-C′), endodermal (D-F′) and ectodermal (G-I′) marker gene expression. Representative embryos from three experiments are shown. (A-D,G) Vegetal views; (E,F) lateral views of bisected embryos, dorsal towards the right; (H) lateral view; (I) animal view. (J) Expression of selected genes in control and H3 MO-injected embryos at stage 10.5 was measured by qRT-PCR. All values were normalized to ornithine decarboxylase (ODC) and plotted relative to the respective transcript levels in control embryos. Error bars indicate s.d. of three independent experiments. *P<0.05 using a two-tailed Student t-test. |