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Display additional annotations [+]
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Supplemental Fig S1. Expression of hhex, sdc4 and fzd7in Xenopus embryo In situ hybridization showing the expression pattern of hhex, sdc4 and fzd7 at late gastrula stage, 0–1 somite stage and 4–6 somite stage. |
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Fig. 6. BMP signaling regulates foregut identity while Wnt/JNK signaling is necessary for both foregut identity and morphology (A–E) Confocal immunostaining of phosphorylated JNK (pJNK) or (F–J) phosphorylated Smad1/5/8 (pSmad1) in the foregut of bisected embryos at stage NF19. Quantification showed in Supplemental Fig. S7 (K–O) Confocal immunostaining of cortical β-catenin as cell membrane marker to show foregut cell morphology at NF19. (P–T) In situ hybridization with hhex in bisected NF19 embryos. The number of embryos with representative phenotype on the left versus total embryos examined on the right. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
bmp2
|
|
laevis
|
NF stage 19
|
prechordal plate
,
endoderm
,
axial mesoderm
,
foregut endoderm
|
bmp4
|
|
laevis
|
NF stage 19
|
mesoderm
,
endoderm
,
foregut endoderm
|
bmp7.1
|
|
laevis
|
NF stage 19
|
mesoderm
,
endoderm
,
foregut endoderm
|
wnt11
|
|
laevis
|
NF stage 19
|
prechordal plate
,
endoderm
,
axial mesoderm
,
endomesoderm
,
foregut endoderm
|
|
|
Supplemental Fig S6. Sdc4 regulates a positive BMP feedback loop to maintain bmp ligand expression. In situ hybridization (A–E) hhex, (F–J) bmp2, (K–O) bmp4+7 or (P–T) wnt11 on bisected NF19 embryos injected with the indicated morpholinos. The number of embryos that exhibited the illustrated phenotype is indicated. |