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Fig. S1 Rpsa depletion does not affect development of other early developmental processes examined. A) Gross morphology of stage 45 control and rpsa depleted embryos. B) Optical coherence tomography of the craniofacial cartilages of stage 45 tadpoles injected with rpsa MO in one cell at the two cell stage, allowing comparison with the uninjected control side of the embryo. No significant differences were detected. C) Expression of nkx2.1, pax2 and hex is unaffected by depletion of rpsa. D) The laterality gene pitx2c is normally expressed in the left lateral mesoderm of rpsa morphants at stage 28 (green arrowheads). |
