|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgg
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
alimentary system
,
intestine
,
liver
|
fgg
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
intestine
,
liver
,
foregut
|
fgg
|
|
laevis
|
NF stage 37 and 38
|
endoderm
,
liver diverticulum
,
midgut
,
foregut
|
ttr
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
liver diverticulum
,
alimentary system
|
ttr
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
alimentary system
|
ttr
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
mesoderm
,
endoderm
,
alimentary system
|
alb
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
liver primordium
|
alb
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
liver primordium
|
alb
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
liver diverticulum
,
alimentary system
|
foxa2
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
alimentary system
,
stomach
,
pancreas
,
lung primordium
,
[+]
|
foxa2
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
alimentary system
,
pancreas
,
lung primordium
,
anterior
,
[+]
|
foxa2
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
brain
,
endoderm
,
liver diverticulum
,
floor plate
,
lung primordium
|
tf
|
|
laevis
|
NF stage 46
to
NF stage 47
|
foregut
,
liver primordium
|
tf
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
alimentary system
,
foregut
|
tf
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
otic vesicle
,
endoderm
,
liver diverticulum
,
tail bud
,
alimentary system
,
[+]
|
tf
|
|
laevis
|
NF stage 46
to
NF stage 47
|
foregut
,
liver primordium
|
tf
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
alimentary system
,
foregut
|
tf
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
otic vesicle
,
endoderm
,
liver diverticulum
,
tail bud
,
alimentary system
,
[+]
|
a2m
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
alimentary system
,
liver primordium
|
a2m
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
intestine
,
liver primordium
|
a2m
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
ectoderm
,
otic vesicle
,
endoderm
,
branchial arch
,
alimentary system
,
[+]
|
sox17a
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
endoderm
,
liver diverticulum
,
alimentary system
,
gall bladder
,
vascular endothelium
,
[+]
|
sox17a
|
|
laevis
|
NF stage 46
to
NF stage 47
|
endoderm
,
alimentary system
,
gall bladder
|
sox17a
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
liver diverticulum
,
alimentary system
,
gall bladder
|
ambp
|
|
laevis
|
NF stage 41
to
NF stage 42
|
endoderm
,
alimentary system
,
liver primordium
|
ambp
|
|
laevis
|
NF stage 29 and 30
to
NF stage 35 and 36
|
endoderm
,
liver diverticulum
|
|
|
Fig. 2. Embryonic expression of liver markers by in situ hybridization. Each indicated probe was tested on three stages: day 7 (stage 46/47) gut tubes in the left column, day 3 (stage 41/42) gut tubes in the centre column and whole tail bud embryos (stages 30–35) in the right column. Light blue 5-bromo-4-chloro-3-indolyl-phosphate colour reactions were used to visualize the specific expression in the day 7 gut tubes. The pancreas tissue is bright white in these preparations. Brown, blue or black colour substrates indicate the specific staining in day 3 gut tubes. All preparations are shown with anterior to the left. lv, liver bud; p, pancreatic bud; gb, gall bladder bud; lu, lung bud. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgg
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
ttr
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
foxa2
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
tf
|
|
xenopus
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
tf
|
|
xenopus
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
a2m
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
sox17a
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
ambp
|
|
laevis
|
NF stage 33 and 34
|
endoderm
,
liver diverticulum
,
alimentary system
|
|
|
Fig. 3. Expression of markers in the liver diverticulum endoderm. (A) Schematic of a tail bud embryo (stage 33) with the line indicating the plane of section through the liver diverticulum (left). The right side shows a typical resulting section with the endoderm indicated in grey. ot, otic vesicle; nt, neural tube; endo, endoderm; ecto, ectoderm; meso, mesoderm; lvd, liver diverticulum. (B) Representative vibritome sections (as illustrated above) of whole-mount embryos hybridized with the indicated probes. Sections from the hex, hnf3β, AMBP, endodermin, fibrinogen and transferrin stained embryos are through the anterior portion of the liver diverticulum at the level of the otic vesicle. Sox17α and transthyretin sections are from the posterior end of the liver diverticulum. All of the markers are expressed in the endodermal layer and excluded from the mesoderm. Note that the dark colour of the outer ectoderm is pigment and not staining. |