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Gene/CloneSpeciesStageAnatomy ItemExperimenter
hhexxenopus gastrocoel roof plate 

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Experiment details for hhex

Homeoprotein hhex-induced conversion of intestinal to ventral pancreatic precursors results in the formation of giant pancrea...

Homeoprotein hhex-induced conversion of intestinal to ventral pancreatic precursors results in the formation of giant pancreata in Xenopus embryos.

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Gene Clone Species Stages Anatomy
hhex.L laevis NF stage 12 gastrocoel roof plate
hhex.L laevis NF stage 13 to NF stage 18 gastrocoel roof plate

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  Fig. 1. vpp1 and hhex display partially overlapping expression in gas- trulating dorsal endoderm and later demarcate ventral pancreatic buds and liver anlagen, respectively. (A) Comparison of vpp1 and hhex expression as revealed by whole mount in situ hybridization analysis with bisected (stages 118) or whole (stages 243) embryos. (1, 2, 5, 6, 9, 10, 13, and 14) Dorsal is toward the right; (172) lateral view, head toward the left. Images 3, 7, 11, and 15 are cartoons drawn representing both the single staining (images 1, 2, 5, 6, 9, 10, 13, and 14) and double staining whole mount in situ hybrid- ization data (Fig. S3B), illustrating vpp1 (purple) and hhex (turquoise) ex- pression. Boxed regions are magnified in 4, 8, 12, and 16, respectively. (B) RT-PCR analysis compares the temporal expression profile of vpp1 and hhex during Xenopus embryogenesis. Ornithine decarboxylase (odc) was used as the RNA loading control.