Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
hhexxenopus neural groove [+] 

Too many results?Too few results?

Experiment details for hhex

Yamamoto TS et al. (2001) Assay

Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling.

Good quality Poor quality
Gene Clone Species Stages Anatomy
hhex.L laevis NF stage 10.5 neural tube , neural groove

Display additional annotations [+]
  Fig. 3. HI-Xmsx-1 induces head organizer marker genes in the ventral endomesoderm. Two ventral blastomeres of 4-cell-stage embryos were coinjected marginally with 100 pg of HI- or TI-Xmsx-1 mRNA and 50 pg of β-galactosidase mRNA as a lineage tracer, then processed for RT-PCR (A) and whole-mount in situ hybridization (B) at stage 10.25. (A) The ventro-vegetal quarter of the embryos injected with the indicated mRNA (top, lanes 1, 2, 3) was dissected at stage 10.25 and RNA was extracted immediately, for early stage, or after being cultured until sibling embryos reached stage 18, for late stage. RNA extracted from each explant was analyzed by RT-PCR. Lane 4 shows the expression of each marker in whole embryos and lane 5 shows the control reactions with no RT step. (B) The injected embryos were stained by red Gal for lineage tracing and then analyzed by whole-mount in situ hybridization for Xotx-2, Xhex and cerberus gene expression. Anterior organizer genes were induced in the ventral endomesoderm. The dorsal blastopore lip is indicated by black arrowheads. Arrows indicate the RNA-injected ventral marginal zone, in which the nuclei of cells are stained red. AS, antisense probe; S, sense probe.
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.12.0


Major funding for Xenbase is provided by grant P41 HD064556