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Fig. 2. hoxa11 and hoxa13 expressions in tadpole blastemas. Blastemas were derived from amputations at the ankle level (A, B, E, F, I and J) or at the knee level (C, D, G, H, K and L). (A, C) hoxa11 expression in 3 days post amputation (dpa) blastema. (B, D) hoxa13 expression in 3 dpa blastema. (E, G) hoxa11 expression in 5 dpa blastema. (F, H) hoxa13 expression in 5 dpa blastema. (I, K) hoxa11 expression in 7 dpa blastema. (J, L) hoxa13 expression in 7 dpa blastema. Note that expression patterns of hoxa11 and hoxa13 during tadpole limb regeneration were similar to those of a developing limb bud (Fig. 1). Lines indicate the estimated amputation planes. Distal is to the top in all figures. Scale bar = 400 μm. |
