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Fig. 3. hoxa11 and hoxa13 expressions in froglet blastemas. Blastemas were derived from amputation at the wrist level (A, B, E and F) or at the elbow level (C, D, G and H). (A, C) hoxa11 expression in 7 days post amputation (dpa) blastema. (B, D) hoxa13 expression in 7 dpa blastema. (E, G) hoxa11 expression in 14 dpa blastema. (F, H) hoxa13 expression in 14 dpa blastema. Note that hoxa11 and hoxa13 were reexpressed in the froglet blastema, but expression domains were never separated. Lines indicate the estimated amputation planes. Distal is to the top in all figures. Scale bar = 400 μm. |