|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxc6
|
|
laevis
|
NF stage 10.5
to
NF stage 11
|
|
hoxc6
|
|
laevis
|
NF stage 11.5
|
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
non-involuting marginal zone
|
hoxc6
|
|
laevis
|
NF stage 12
to
NF stage 12.5
|
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
dorsal marginal zone
,
[+]
|
tbxt
|
|
laevis
|
NF stage 10.5
to
NF stage 11
|
marginal zone
,
dorsal marginal zone
,
involuting marginal zone
,
involuted dorsal mesoderm
,
upper blastopore lip
|
hoxd1
|
|
laevis
|
NF stage 10.5
to
NF stage 11.5
|
neuroectoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
[+]
|
hoxd1
|
|
laevis
|
NF stage 12
to
NF stage 12.5
|
mesoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
[+]
|
hoxb4
|
|
laevis
|
NF stage 10.5
|
|
hoxb4
|
|
laevis
|
NF stage 11
to
NF stage 11.5
|
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
|
hoxb4
|
|
laevis
|
NF stage 12
to
NF stage 12.5
|
dorso-lateral marginal zone
,
circumblastoporal collar
,
posterior
|
hoxc9-like
|
|
laevis
|
NF stage 10.5
to
NF stage 11
|
|
hoxc9-like
|
|
laevis
|
NF stage 11.5
to
NF stage 12
|
dorso-lateral marginal zone
|
hoxc9-like
|
|
laevis
|
NF stage 12.5
|
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
dorsal marginal zone
,
circumblastoporal collar
,
[+]
|
|
|
Fig. 1. Spatial and temporal Hox expression during gastrulation. Results for Hoxd-1, Hoxb-4, Hoxc-6, Hoxa-7 and Hoxb-9 are shown, Hoxa-1, Hoxb-1 and
Hoxb-7 also fit this sequence (not shown), Hoxd-13 expression did not begin before the end of gastrulation (stage 15, not shown). (A) WISH for five Hox genes
at five different stages. Vegetal views, dorsal up. (B) Diagram showing the onset of the temporally colinear expression of five different Hox genes as analysed
with WISH (red) and PCR (blue). (C) Localisation of Hoxd-1 expression in mesoderm and ectoderm. Embryos cut into halves across the dorsolateral blastopore
lip at stages 10.5 and 11. One half analysed with Xbra, the other with Hoxd-1. The Xbra expression domain is outlined. The initial Hoxd-1 expression is located
within the mesoderm. At stage 11, it is expanded into the presumptive neurectoderm (arrowheads). |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxc6
|
|
laevis
|
NF stage 12
|
neuroectoderm
,
dorsal marginal zone
,
non-involuting marginal zone
|
hoxc6
|
|
laevis
|
NF stage 12.5
|
neuroectoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
[+]
|
hoxc6
|
|
laevis
|
NF stage 26
|
spinal cord
,
presomitic mesoderm
,
pronephric duct
,
tail bud
,
trunk
,
[+]
|
hoxd1
|
|
laevis
|
NF stage 11
|
neuroectoderm
,
dorsal marginal zone
,
involuting marginal zone
,
non-involuting marginal zone
|
hoxd1
|
|
laevis
|
NF stage 12.5
|
circumblastoporal collar
,
non-involuting marginal zone
,
posterior
|
hoxd1
|
|
laevis
|
NF stage 26
|
spinal cord
,
presomitic mesoderm
,
mandibular arch
,
branchial arch
|
hoxc9-like
|
|
laevis
|
NF stage 12.5
|
involuted dorsal mesoderm
,
involuted ventral mesoderm
|
hoxc9-like
|
|
laevis
|
NF stage 26
|
spinal cord
,
tail bud
,
posterior neural tube
,
lymph vessel
,
trunk
,
[+]
|
|
|
Fig. 2. Hox expression in ventralised and dorsalised embryos. (A) Hoxc-6 in embryos ventralised with UV. WISH (vegetal views of stages 10.5 to 12.5,
lateral view of stage 26). Similar results were obtained for Hoxd-1, Hoxb-4, Hoxa-7 and Hoxb-9 (not shown). (B) Diagram showing the onset of Hox
expression (detected with WISH) in ventralised (dark blue) and control embryos (light blue). In ventralised embryos, the temporally colinear sequence is still
present. (C) Mesodermal Hox expression (WISH) in ventralised embryos. Embryos cut through dorsolateral blastopore lips in controls and the corresponding
region in ventralised embryos. Hoxd-1 at stage 11, Hoxc-6 at stage 12, Hoxa-7 at stage 12.5. The white line indicates Brachet’s cleft, separating involuted
mesoderm from overlying ectoderm. Neurectodermal Hox staining in control embryos (C, arrowheads) is missing in ventralised embryos (C’). (D) Hox
expression is absent in embryos that were dorsalised with LiCl. Dorsalised embryos (LiCl) and controls (con) at stage 12.5 (vegetal views) and stage 26
(lateral views). Results of a WISH for Hoxd-1, Hoxc-6 and Hoxb-9. Analysis of Hoxb-4 and Hoxa-7 showed similar results (not shown). Arrowheads
indicate the anterior Hox expression boundary. |