| |
Fig. 3. Localization of Hoxb gene expression on sections. Transverse (A-E, G) and sagittal (F) sections of whole embryos hybridized with Hoxh gene probes. Only the dorsal structures are shown. The hybridization signal is blue and the red color is a nuclear counterstain. (A) Strong Hoxb1 expression in the neural crest and weaker expression in the forming neural tube at stage 17. (B) Hoxb-1 expression in rhoxnbomere 4 at stage 24. Note the unstained floorplate of the neural tube. (C) Hoxb-3 expression in the neural crest and future neural tube at stage 17/18. (D) Hoxb-3 expression in the hindbrain at stage 27. The epidermal staining is nonspecific. This section was not counterstained with neutral red. (E) Hoxb-4 expression in the future neural tube of a stage 17 embryo The somites are also weakly stained (F) Sagittal section of a stage 32 embryo which had been hybridized with the Hoxb-4 probe, showing somite staining. (G) Hoxb-5 staining in the spinal cord of a stage 28 embryo. Abbreviations: nc, neural crest; n, notochord; ar, archenteron cavity; s, somites; e, ear anlage.
|
