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FIG. 5. Induction of HoxB9 RNA by dorsal mesoderm was not affected in animal caps expressing XFD. HoxB9 is present in the posterior
neural tube of a stage 26 embryo by whole-mount in situ hybridization (between arrowheads in A). Some background staining was observed
in the head. (B) Expression of HoxB9 in uninjected ectoderm recombined with dorsal mesoderm (arrowhead denotes condensed staining and
arrow denotes diffuse staining). (C) Expression of HoxB9 RNA in XFD-expressing ectoderm recombined with dorsal mesoderm (arrowhead
denotes condensed staining and arrow denotes diffuse staining). HoxB9 expression was not seen in untreated animal caps as seen in D
(section). (E and F) The expression of HoxB9 (E, arrowhead) is not found in the RLDX-labeled dorsal mesoderm (F, arrowhead). (G) HoxB9
is present in the ectoderm (G, arrowhead) and not in the RLDX-labeled mesoderm (H, arrowhead). Scale bar, 150 mm. |