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Supplementary Figure 3 – Posterior neural marker gene expression is reduced at early stages in sall morphants
Splice-blocking sall1 and sall4 morpholinos induce partial loss of posterior neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stage 13 using markers hoxb9 (A-D) and hoxd10 (E-H); dorsal view, anterior up. Splice-blocking sall1 and sall4 morpholinos were injected unilaterally into the embryos shown here. Representative images are shown. Asterisks in images indicate injected side; arrows indicate partial loss of expression. Expression of hoxb9 in sall morphants at later stages is shown in Figure 2 and Supplementary Figure 2.
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Supplementary Figure 2 – Neural patterning and morphogenesis are disrupted by translation-blocking sall morpholinos
Translation-blocking morpholinos induce changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-L) and 23 (M-O) using markers otx2, krox20, and hoxB9 in combination (A-C), pax3 (D-F), pax6 (G-I), sox2 (J-L), and tubb2b (M-O); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Compare to Figure 2, splice-blocking morpholinos; see Figure 2 for quantification of neural marker phenotypes in all sall morphants.
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