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hoxb9xenopus   

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Experiment details for hoxb9

Serine Threonine Kinase Receptor-Associated Protein Deficiency Impairs Mouse Embryonic Stem Cells Lineage Commitment Through...

Serine Threonine Kinase Receptor-Associated Protein Deficiency Impairs Mouse Embryonic Stem Cells Lineage Commitment Through CYP26A1-Mediated Retinoic Acid Homeostasis.

Gene Clone Species Stages Anatomy
hoxb9.L laevis NF stage 19 to NF stage 20 spinal cord

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  Figure 6. STRAP knockdown influences anterior‐posterior neural patterning and impairs the body axis and eye de‐ velopment in early Xenopus embryogenesis.  A. Strap transcript was detected in the entire blastopore (a), early neu‐ rulation (b), late neurula stage (c), and the tailbud and the tadpole stages (d and e, respectively). B. 50 ng Strap‐MO was injected into the animal regions of two‐cell‐stage embryos and resulted in defects in the tadpole stage. C. Strap RNA (0.20 ng) was injected alone or with 25 ng Strap‐MO unilaterally into two‐cell‐ stage embryos. The embryos were stained with the β‐Gal substrate Red‐Gal. D. 50 ng Strap‐MO was injected unilaterally into two‐cell‐ stage embryos. The embryos were examined at neurula stages 17‐18 by ISH for neural markers. E.  50 ng Strap‐MO was injected into the animal regions of two‐cell‐stage embryos. Animal caps were dissected at blastula stage 9 and cultured until neu‐ rula stages 19‐20. RNA was then extracted for RT‐qPCR analysis of marker expression. Bars represent mean ±s.d., n=3. * P<0.05,  **P<0.01, when compared with the control.