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hoxb9xenopus nucleus 

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Experiment details for hoxb9

Wright CV et al. (1990) Assay



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Gene Clone Species Stages Anatomy
hoxb9.S laevis NF stage 24 nucleus
hoxb9.S laevis NF stage 25 to NF stage 26 nucleus

  Fig. 5. Most neuronal precursor cells express XlHbox 6 in stage 24 spinal cord. Dorsal is uppermost in all panels. (A) Section at high power magnification immunostained with anti-XlHbox 6 antibodies. (B) Same section counterstained with Hoechst 33258 viewed under dark field illumination. Note that nearly all nuclei appear to be darkly stained in panel A and that the Hoechst fluorescence in these nuclei is quenched by the immunostaining reaction product. (C) XlHbox 6 expression in lateral plate mesoderm. Transverse section of stage 25-26 tadpole decorated with XlHbox 6 antibodies and overstained to show more clearly the less intense staining in the lateral plate mesoderm nuclei. Abbreviations: ecto, ectoderm; ec, ependymal canal; sc, spinal cord; myo, myotome; LPM, lateral plate mesoderm; endo, endoderm; arch, archenteron; no, notochord, remains of blastopore.

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Gene Clone Species Stages Anatomy
hoxb9.S laevis NF stage 27 nucleus

  Fig. 6. Distribution of XlHbox 6 antigens in the spinal cord of stage 27 tadpole. A transverse section was (A) immunostained with XlHbox 6 antibodies and (B) counterstained with Hoechst 33258. At this stage of development, the spinal cord starts to display a degree of differentiation into defined layers. Now, not all of the spinal cord nuclei express XlHbox 6 antigens. The more peripheral nuclei which probably correspond to differentiated primary neurons are no longer stained. Immunostaining is confined to the entire inner layer (ependymal layer) and a few cells that have migrated laterally away from this mitotic zone. Note the nuclei that are much less intensely immunostained in the lateral plate mesoderm indicated by arrowheads. Abbreviations: ec, ependymal canal; sc, spinal cord; no, notochord; endo, endoderm; myo, myotome; ecto, ectoderm; LPM, lateral plate mesoderm.

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Gene Clone Species Stages Anatomy
hoxb9.S laevis NF stage 37 and 38 nucleus

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  Fig. 4. Expression of XlHbox 6 antigens is limited to posterior central nervous system. (A) A tailbud embryo (stage 25) was paraffin sectioned and immunostained for XlHbox 6 antigens. Black areas in the spinal cord represent immunostaining. This embryo was curled so that an anterior section through the brain is at left and a section through the spinal cord is at right. (B) Expression of XlHbox 6 in a stage 38 spinal cord. This is a slightly skewed, or oblique, lateral section. The periodic row of black dots (arrowheads) represents staining in a very few nuclei at the ependymal surface of the spinal cord. Abbreviations: ec, ependymal canal; sc, spinal cord; myo, myotomes; hb, hindbrain; no, notochord; endo, endoderm; LPM, lateral plate mesoderm; df, dorsal fin.
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