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Fig. 4. AGN incubation affects early expression of Hoxd-1 in the neuro- ectoderm but not in the underly- ing mesoderm. Whole-mount in situ hybridizations on Xenopus laevis em- bryos. Hoxd-1 expression at st. 11 A-C) or st. 12.5 (D-F). Embryos were ncubated with 10-6 M AGN (A,D), 10-6 M RA (C,F) or not treated (0.1% DMSO) (B,E). After photographing the em- bryos were cut along the indicated dashed line and a lateral view of the cut surface is shown next to the right of each embryo. Arrows in (D,E) point to the faint mesodermal expression remaining at that stage (mostly non- involuted mesoderm). Hoxc-6 expres- sion on stage 12 embryos (G-I). Treat- ment with 10-6 M AGN (G), 10-6 M RA (I) or not treated (H). |
