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Fig. 5. Specificity of X-Delta-2 morpholinos. Two non-overlapping morpholinos were injected in the LHS of the embryos and the Hoxc6 expression analysed at gastrula stage. The Hoxc6 expression is shown for NIC (A), control MO (B: 100%, n=21), MO1 (C: 88%, n=26) and MO2 (D: 100%, n=35). Overexpression of X-Delta-2 mRNA (E: 100%, n=26) and rescue of X-Delta-2 MO2 with X-Delta-2 mRNA (F: 66% rescued, n=30) are also shown. Embryos are shown from the vegetal side with dorsal to the top. Both MOs knocked down Hoxc6 expression in the LHS and co-injection of X-Delta-2 mRNA is able to rescue the Hoxc6 expression. Arrowheads indicate the injected side of the embryo. |
