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FIG. 7. Transcript accumulation in the adult spinal cord. Darkfield photographs of transverse sections of the adult spinal cord. (a) and (b) Are at low magnification (9X), lshowing the entire spinal cord. (c) and (d) Are at higher magnification (47x), showing a detail focused on the left half of the gray matter. Tissue was dissected from an adult female and fixed in methanol, as described for embryos. Likewise, in situ hybridization was carried out in the same way as described for embryos. (a) and (c) Represent controls stained with Giemsa and photographed in darkfield to show cellular details of the sections. In the ventral horn the large motoneurons (m) can be identified. The rest of the gray matter shows groups of neurons (n, with pointers) with different shapes. (b) Shows a section adjacent to (a) hybridized with the Xeb1 antisense probe in the same way as was performed in embryo sections, and exposed for 40 days. Brackets show the region which is magnified in (d). (d) shows the bracketed region, enlarged from (b), of half of the spinal cord gray matter. Groups of neurons (n, pointers) display signal in the lateral field [compare (d) with (e) to localize the cell position in the same half of the spinal cord). (e) Represents a section through the medulla oblongata hybridized concomitantly with the section shown in (a). No signal can be observed in the section. |
