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Figure 1. Temporal and spatial expression pattern of the Hoxc6 isoforms. A: Structure of Hoxc6 isoforms in Xenopus laevis. Hoxc6 isoforms derive from 2 different promoters. The distal promoter (PR I) leads to a 2.2-Kb precursor transcript, coding for the short form (SF) protein of 152 aa. The proximal promoter encodes a 1.8-Kb transcript leading to the long form (LF) protein of 234 aa. Black boxes indicate the open reading frame, grey boxes are the homeodomain. Red bars indicate the binding sites of the morpholino-oligonucleotides. The blue and green arrows show the primer sites that served for the RT-PCR and to generate the probes for in situ hybridization. B: Temporal expression pattern of Hoxc6 isoforms. RT-PCR was carried out using embryos at the indicated stages from 1 to 27. H4 is used as a loading control; -RT is shown for stage 12 RNA. C-K: SF expression patterns from beginning of gastrulation until tadpole stages. Stages are indicated by the numbers. L-T: LF pattern from beginning of gastrulation until tadpole stages. The arrowheads in K and T indicate the location of the pronephric anlage. The squared brackets indicate the different distances between the anteriormost border of expression of each isoform and the otic vesicle area. |
