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Supplementary Figure 3 – Posterior neural marker gene expression is reduced at early stages in sall morphants
Splice-blocking sall1 and sall4 morpholinos induce partial loss of posterior neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stage 13 using markers hoxb9 (A-D) and hoxd10 (E-H); dorsal view, anterior up. Splice-blocking sall1 and sall4 morpholinos were injected unilaterally into the embryos shown here. Representative images are shown. Asterisks in images indicate injected side; arrows indicate partial loss of expression. Expression of hoxb9 in sall morphants at later stages is shown in Figure 2 and Supplementary Figure 2.
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