| |
Fig. 6. Bicaudal-C and cilia formation. (A–D) Whole mount in situ hybridization of uninjected (A–C) or xBic-C-MO1 + 2-injected Xenopus embryos (D) at stage 36 using (A) FoxJ1, (B) Axonemal Dynein Heavy Chain 9 (ADHC9) and (C, D) polaris. (E–J′) Immunocytochemistry using anti-acetylated α-tubulin antibody (red) on uninjected control, xBic-C-MO1 + 2 and xPol-MO-injected embryos at stage 42. (E, E′, H, H′) Ciliated cells of the epidermis; (F, F′, I, I′) cilia of the nephrostomes; (G, G′, J, J′) individual primary cilia present on the cells of the pronephric duct. (K, K′) Immunocytochemistry using anti-acetylated α-tubulin (red) and anti-NBC1 (green) antibodies on uninjected control and xBic-C-MO1 + 2-injected embryos at stage 42. Images were obtained by confocal microscopy; nuclei were counterstained with DAPI (blue). |
