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il6stxenopus   

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Experiment details for il6st

Leukemia inhibitory factor signaling in Xenopus embryo: Insights from gain of function analysis and dominant negative mutant...

Leukemia inhibitory factor signaling in Xenopus embryo: Insights from gain of function analysis and dominant negative mutant of the receptor.

Gene Clone Species Stages Anatomy
il6st.L laevis NF stage 35 and 36 to NF stage 37 and 38 kidney , pharyngeal region

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  Fig. 2. Temporal and spatial expression ofXenopus laevis lifand lif receptors encoded genes. (A) RT-PCR analysis of embryo from stage 2 to stage 33. (B) RT-PCR analysis of dissected parts of stage 11 embryo. AC, animal cap; Do, dorsal mesoderm; Em, total embryo; En, endoderm; Ve, ventral mesoderm. Chordin and wnt8 expression is used as control. (C) RT-PCR analysis of adult tissues. Br, brain; He, heart; Ki, kidney; Li, liver; Lu, lung; Mu, skeletal muscle; Ov, ovary; Sk, skin; Sp, spleen; St, stomach; Te, testis. Ornithine decarboxylase (odc) and ribosomal protein L8 (rpl8) gene expression was used as control. - Control without reverse transcription. (D) In situ hybridization on stage 37/38 embryo with lif, lifr and il6st antisense probes. Ba, branchial arches; ki, embryonic kidney; nt, neural tube; ot, otic vesicle; so, somites.