| |
Fig. 5. Expression of xefiltin (A), XNIF (B) and NF-L (C) transcripts
in the retina of juvenile X. laevis revealed by in situ hybridization
with digoxigenin labeled anti-sense cRNA probes. As a negative
control, one section was hybridized with a labeled cRNA probe
transcribed in the sense orientation of xefiltin (D). In retinal ganglion
cells (examples indicated by unlabeled arrowheads), the level of
xefiltin mRNA was high, whereas that of XNIF was moderate, and
that of NF-L was low. The XNIF probe also occasionally labeled
neurons scattered along the inner margin of the inner nuclear layer
(arrowhead labeled ‘‘i’’ in B). r, retinal ganglion cell layer; i, inner
nuclear layer; p, photoreceptor layer. The scale bar 5 50 μm in D,
applies to all panels. |
