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Fig. 4. Validation of microarray data. (A,B) Initial confirmation of the data by RT-PCR analysis of selected genes found to be enriched in either the dorsal or ventral bud fraction. (C-E) Dorsal-specific localization of brunol1, insm1 and secretogranin III in isolated liver/pancreas tissue at NF40, except for secretogranin III, which is at NF44. Expression in all three instances is punctate. (F-H) Ventral pancreas-specific expression of tm4sf3, pkc-alpha and agr2 at NF40/42. Expression is localized to a small region in the ventral pancreas, where the two ventral pancreatic buds fuse, and to where the bile duct emerges. Broken lines in C and F indicate the boundary of dorsal and
ventral pancreas. |
