Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
insm1xenopus endocrine cell 

Too many results?Too few results?

Experiment details for insm1

Oropez D and Horb M (2012) Assay

Transient expression of Ngn3 in Xenopus endoderm promotes early and ectopic development of pancreatic beta and delta cells.

Good quality Poor quality
Gene Clone Species Stages Anatomy
insm1.L laevis NF stage 32 endocrine cell

Display additional annotations [+]
  Figure 6. Insm1 and Rfx6 are necessary for ectopic beta cell promotion by Ngn3-GR. In situ hybridizations for insulin expression in all panels. Ngn3-GR mRNA (1,800 pg) was coinjected with a control mismatch morpholino (mMO) or morpholinos targeting insm1 or rfx6 mRNA, activated with Dex for 4 h and fixed at Stage 32. (a,b) Insm1 mMO (20 ng) had no effect on endogenous insulin expression in either ex (n = 8/8) or +Dex (n = 12/17) treated embryos. (c,d) Injection of Insm1 MOs (2 morpholinos, 20 ng each) abolished endogenous insulin expression in ex embryos (n = 7/7) as well as ectopic insulin expression in +Dex embryos (n = 15/15). (e,f) Rfx6 mismatch morpholino (25 ng) did not affect endogenous or ectopic insulin expression in ex (n = 9/9) or +Dex embryos (n = 19/41). (g,h) Antisense Rfx6 morpholino (25 ng) inhibited endogenous insulin expression in −Dex embryos (n = 33/33) as well as ectopic insulin expression in +Dex embryos (n = 46/46).
Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556