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Fig. 5. Optic pathway apparently unaffected by MMP inhibition. Embryos were exposed at stage 31 to either a control solution (A,C,E,G,I,K) or 25 μM SB-3CT (B,D,F,H,J,L). At stage 37/38, embryos were fixed. (A-D) Embryos were processed as wholemounts for in situ hybridization for Xenopus slit mRNA. Vibratome sections (50 μm) were cut. The ventral diencephalon at the level of the optic chiasm is represented in A,B, and eye sections are shown in C,D. (E-H) Immunoreactivity for the glial cell marker 3CB2. (G,H) Higher power views of E,F, showing the optic nerves and the optic chiasm in the ventral diencephalon. (I,J) Islet 1 immunoreactivity showing label in the ventral region of the diencephalon adjacent to the optic chiasm, and labelling of RGCs and cells in the inner nuclear layer in the retina. (K,L) GABA immunoreactivity showing labelling of amacrine cells in the inner nuclear layer, and a population of neurons in the ventral diencephalon. L, lens; nc, notochord; ap, anterior lobe of the pituitary body; nr, neural retina; PE, pigment epithelium; oc, optic chiasm; on, optic nerve, RGCL, RGC layer; Am, amacrine cells; INL, inner nuclear layer; ONL, outer nuclear layer; Di, diencephalon. D, dorsal; V, ventral. Orientation in A is for A,B,E,L, and orientation in C is for C,D. Scale bars: in A, 50 μm for A,B; 25 μm for C,D; in E, 50 μm for E,F,I,L; 25 μm for G,H. |
