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Supplemental Figure 1. The XMeis3 MO inhibits neuron and neural crest cell fates in later tadpole stage embryos. A. Islet-1 (Xisl-1) expression is inhibited in XMeis3 morphant embryos in comparison to controls embryos (upper panel), 7.5 ng XMeis3 MO (center panel) or 10 ng XMeis3 MO (lower). Xisl-1 expression was highly inhibited in over 85% of the morphant embryos (n = 69). XMeis3 MO was injected at the one-cell stage. At tadpole stages, Xisl-1 marks a subset of cranial neurons (upper panel). Note that Xisl-1 expression in the heart region of morphant embryos is fairly normal despite the strong neural phenotype. B. Tanabin/nestin expression is inhibited in XMeis3 morphant embryos. At tadpole stages, tanabin/nestin is a marker for cranial nerve cells (upper panel). Expression is ablated in morphant embryos (lower panel). C. Melanocytes are lost in XMeis3 morphant embryos. Compare controls embryos (left panel), to morphants, 10 ng XMeis3 MO (center panel) or 12.5 ng XMeis3 MO (right panel). Melanocyte formation was highly inhibited in over 90% of the morphant embryos (n = 50). XMeis3 MO was injected into one blastomere at the two-cell stage. |
