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Fig. 6. Runx1-deficient embryos downregulate N-Tub, Pak3 and Islet1. (A) Embryos were injected in one blastomere at the 2-cell stage with Runx1MO, Aml1MO or Runx1SMO and analyzed at stage 15 for the expression of Ngnr1, N-Tub, Pax3 and Islet1. The RB expression domain of N-Tub, Pak3 and Islet1 is reduced while Ngnr1 expression is expanded (arrows). In all panels the injected side is on the right. Dorsal view anterior to top. (B) Transverse sections of representative Runx1MO-injected embryos. The expression of N-Tub is lost in RB progenitors while N-Tub expression in primary motor neurons precursors is unaffected (arrow heads). Ngnr1 expression is expanded (arrows). The injected side is on the right. Dorsal to top. no, notochord; so, somites. |
