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Fig. 7. Ngnr1 expression is not sufficient to activate Runx1 expression. (A) Embryos at the 2-cell stage were injected in one blastomere with 0.5 ng of Ngnr1-GR mRNA. Embryos were subsequently incubated with dexamethasone at early (+ Dex 10.5) or late (+ Dex 12.5) gastrula stages, and fixed at stage 15 for detection of Runx1, N-Tub or Pak3 by whole mount in situ hybridization. N-Tub and Pak3 are dramatically upregulated while the Runx1 expression domain is only marginally affected. The injected side is on the right. Dorsal view anterior to top. (B) At the tailbud stage (stage 27) these embryos show ectopic Kv1.1 and Islet1 expression in the ectoderm, independently of any upregulation of Runx1. Lateral views dorsal to top. Control and injected sides of the same embryo are shown for comparison. |
