| |
Fig. 4. Histological analysis of molecular markers expressed before
and during fusion of the pancreatic rudiments. A) Stained sagittal
section of a stage 39 tadpole showing both the dorsal and ventral
pancreas. B) Close-up of the bracketed area in A. C) Islet1 RNA expression
in a sagittal section of a stage 39 tadpole. D) Islet1 RNA expression
in a transverse section of a stage 37 tadpole. E) Insulin protein expression
in a transverse section of a stage 39 tadpole. F) NeuroD RNA
expression in a transverse section of a stage 39/40 tadpole. G) Pax6
RNA expression in a transverse section of a stage 39/40 tadpole. The
section in F is more anterior than in G. Abbreviations: a, aorta; dp, dorsal
pancreas; h, heart; int, intestine; lv, liver; nc, notochord; nt, neural tube;
oe, oesophagus; ph, pharynx; pn, pronephros; s, somite; st, stomach; vp,
ventral pancreas. |
