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Fig. 3. Committed progenitor cells continue to differentiate normally following ND. (A) Many cells labelled with EdU 20 minutes prior to ND were found in the differentiated central retina after a 3-day chase period. n=3, 12 embryos/condition. (B) These cells increased in number in the ND condition compared with control retinas. n=3, 12 embryos/condition. Error bars show 95% confidence intervals; *P≤0.05 (unpaired t-test). (C,D) High magnification of the central retina, which contains differentiated cells. Some EdU-labelled cells were also positive for the neuronal marker Islet1, which marks specific subsets of amacrine and bipolar cells in the inner nuclear layer of the differentiated retina. n=3, 12 embryos/condition. Scale bars: 40 μm (C); 10 μm (D). |
