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Fig. 3. Cep4l induces specifically primary sensory neurons. ((A)–(H)) Cep4l overexpression induces ectopic lateral neurons. ((A)–(D)) Unilateral β-gal alone, 50 pg (red). ((E)–(H)) β-gal+250 pg cep4l. Neuronal markers are pan-neuronal tubb2b ((A) and (E)), Rohon–Beard sensory runx1 ((B) and (F)), sensory and motor islet1 ((C) and (G)), interneuron pax2 ((D) and (H)). Brackets in ((E)–(G)) indicate unilaterally expanded lateral neuron region in cep4l-injected embryos. (M) Quantification of the mean difference in marker positive neuron populations, injected–uninjected sides. Error bars indicate 95% CI. |