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Figure 7. Overexpression of NM23 activates gliogenesis. (A-D) Retinal section at stage 41 after lipofection with GFP (A), NM23-X1 (B), or NM23-X4 (C). A representative image of Müller glial cells in the retina transfected with NM23-X4 and GFP (D). (E-P) Immunostaining of NM23-X4 lipofected retinas with cell specific markers. (E-G) Müller glia staining using R5 antibody: (E) green fluorescent protein (GFP); (F) R5; (G) merged. (H-J) anti-CRALBP staining of Müller glial cells: (H) GFP; (I) anti-CRALBP; (J) merged. (K-M) Ganglion cell staining against islet-1 using 39.4D5 antibody: (K) GFP; (L) 39.4D5 staining; (M) merged. (N-P) Photoreceptor staining with anti-calbindin: (N) GFP; (O) calbindin staining; (P) merged. (Q) Distribution of cell types in the retina after lipofection with NM23 family members. |
