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Figure 2. Early Fgf signal is not required for the specification of many retinal cell types. A–L: 12-μm transverse cryosections of Stage-40 embryos treated for 5 hr at Stage 12 with control solution (A–C and G–I) or with 150–200 μM SU5402 (D–F and J–L) imunolabelled with anti-Islet1 (B, E) to label retinal ganglion and amacrine cells, anti-Otx (C, F) to label photoreceptor and bipolar cells, anti-rhodopsin (H, K) to label rod photoreceptors, and anti-Calbindin (I, L) to label cone photoreceptor cells. Fluorescent secondary antibodies were used for visualization. Dorsal is up in all images. M–O: The numbers of cells in the dorsal retina (portion of the retina dorsal to the optic nerve head), ventral retina, and the total number of cells for the various immunostains were quantified in central retinal sections. The dorsal/ventral axis, inner nuclear layer (inl), lens (L), outer nuclear layer (onl), and retinal ganglion cell layer (rgc) are indicated. Arrows point to the optic nerve head. Values in graphs are mean ± standard error of the mean (SEM), and average values were compared where indicated using unpaired t-tests. (*P ≤ 0.05, ***P < 0.001; n=8 for controls and n=7 for SU5402 for all Otx and Islet labeled groups, and n=4 for all Calbindin-labeled groups). PR Otx and Bp Otx refers to Otx-positive cells in the outer and inner nuclear layers, respectively. Am Isl and RGC Isl refers to Islet1 positive cells in the inner nuclear and retinal ganglion cell layers, respectively.Download figure to PowerPoint |
