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Experiment details for isl1

Simon E et al. (2017) Assay

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration.

Gene Clone Species Stages Anatomy
isl1.S laevis NF stage 14 to NF stage 20 neural crest , preplacodal ectoderm

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  Figure S1. Vgll3 does not co-localise with trigeminal placode markers. (A) Wholemount ISH for vgll3 and trigeminal placode genes islet1, neuroD, pax3 and foxi1c. (B) Whole-mount ISH for trigeminal placodal genes islet1, neuroD, pax3 and foxi1c. Blue arrow indicates the position of vgll3 expression domain. All staining are visualized in blue. Analysed embryos are from stage 14 to stage 20 depending on the trigeminal markers used. All views are dorsal-anterior.

Gene Clone Species Stages Anatomy
isl1.S laevis NF stage 18 neural crest , preplacodal ectoderm
isl1.S laevis NF stage 19 neural crest , preplacodal ectoderm

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  Fig. 2. Vgll3 knockdown or overexpression impair trigeminal placode and nerve formation. (A) Embryos injected with v3MO or v3MOsplicing (v3LMOsp and v3SMOsp, 20 ng each) exhibit a reduced expression of islet1, neuroD and N-tubulin in the trigeminal placodes (arrowhead). (B) Overexpression of increasing amounts of vgll3 mRNA reduces islet1 and N-tubulin expression in stage 19 embryos. Double ISH shows no prominent overlapping staining between vgll3 (red) and N-tubulin (blue) (arrow). Injected side (*) was traced by lacZ staining. Gene expression were assayed by ISH. Arrowhead indicates the trigeminal placodes. (C) Quantification of results. Images on the top of bars indicated v3LMOsp defects rescued with increasing amounts of vgll3 mRNA injections. Three independent experiments were performed. The number of embryos analysed is indicated on the top of each bar. Views are dorsal-anterior excepted for lateral views for stage 28 embryos.