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Supplement Fig. 4 Mesoderm formation and cardiac specification are not dependent on Dkk1.
A. Expression of mesoderm marker tbxt is not affected upon Dkk1 knockdown. tbxt expression was analyzed at stage 10 embryos injected with Dkk1 MO or Control MO at stage 4. Red dotted circles indicate the location of the cardiac progenitor cell population induced at the onset of gastrulation. Quantification is shown in B. C. Expression of early cardiac markers is not altered upon Dkk1 depletion. Gene expression was analyzed at stage 13 embryos injected with Dkk1 MO or Control MO at stage 4. Quantification is shown in D. Mean values with standard errors are given. n = number of independent experiments, N = total number of embryos analyzed.
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Fig. 5. The expression of differentiation cardiac markers is downregulated upon loss of Dkk1 and can be rescued by co-injection of Dkk1 MO and human DKK1. A. Front view embryos of stage 28 showing reduced expression of cardiac marker genes on MO injected side (black arrow). Control MO or Dkk1 MO was injected unilaterally at the dorsal-vegetal site in embryos at stage 4. Un-injected side served as internal control. Data quantification is shown in B. C. Co-injection of Dkk1 MO and human DKK1 restore the reduced expression (black arrow) of markers as shown in stage 28 embryos of front view. Data quantification is presented in D. White dotted lines indicate the cement gland. Mean values with standard errors are given. n = number of independent experiments, N = total number of embryos analyzed. *p < 0.05, **p < 0.01 with Mann-Whitney rank sum test. |
