|
|
| |
Figure 8: The development of Isl1-positive cardiac progenitor cells is largely
unaffected in Kmt2d morphants. Embryos were injected with 2.5 ng MO Kmt2d (or
2.5 ng 5 bp mismatch control) or 5 ng splice MO (or 5 ng control MO) in combination
with 100 pg LacZ RNA in one dorsal blastomere at the 4-cell stage. At early neurula
stage 14/15 embryos were analyzed for Isl1 expression by in situ hybridizationA
Uninjected control embryo; left (anterior view), right (dorsal view) of the same
embryo. B Mismatch MO injected embryo. C Kmt2d MO injected embryo. D Graph
summarizing three independent experiments. Standard errors of the means and
numbers of analyzed embryos are shown for each condition; n.s. not significant in a
Student’s t-test. E Uninjected control embryo. F Control MO injected embryo. G
Splice MO injected embryo. H Graph summarizing three independent experiments.
Standard errors of the means and numbers of analyzed embryos are shown for each
column; n.s. not significant in a Student’s t-test. |
|
Display additional annotations [+]
|
| |
Figure 6: The expression of first and second heart field markers is affected by
Kmt2d loss of function. Embryos were injected with 2.5 ng MO (Kmt2d or 5 bp
mismatch control) in combination with 100 pg LacZ RNA in one dorsal blastomere at
the 4-cell stage and analyzed by Tbx20 or Isl1 in situ hybridization. A Schematic
drawing of Tbx20 expression in the first heart field at stage 29. B-E Embryos
analyzed by Tbx20 in situ hybridization shown from the ventral side; asterisks
indicate the injected side. B Uninjected control embryo. C Embryo injected with
mismatch control MO. D,E Kmt2d morphant embryos showing a reduction as well as
a posterior shift of Tbx20 expressing cells on the injected side. F Graph summarizing
three independent injection experiments, standard errors of the means and numbers
of analyzed embryos are indicated for each column. *** P-value in a Student’s t-test <
0.001. G Schematic drawing of Isl1 expression in the second heart field at stage 29.
H-K Embryos analyzed by Isl1 in situ hybridization. H Uninjected control. I Mismatch
control. J,K Kmt2d morphants with reduced expression of Isl1. L Graph summarizing
three independent experiments, s.e.m as well as numbers of analyzed embryos are
indicated. ** P-value in a Student’s t-test < 0.01. |
