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Figure 4. The effect of Hoxc6 knockdowns on formation of primary neurons and cranial nerves. Markers specific for primary neurons (N-tubulin, Xisl-1) and for cranial nerves (Xisl-1) were analyzed at neurula and tadpole stages by in situ hybridization upon Hoxc6 depletion. Shown are uninjected (uninj.) embryos, and embryos injected with ctMO, MO2, MO3, or a combination of MO3 with an MO-insensitive mRNA for LF, as indicated above the photographs. A-E: Analysis of the neuronal marker N-tubulin. Arrowheads indicate ectopic N-tubulin expression. F-J: Expression of Xisl-1 at a neurula stage for the same set of injections. No ectopic expression was found. K-O: Expression of Xisl-1 in the head of a tadpole. Numbers in K indicate the different branchial arches and the corresponding cranial nerves. |
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Figure 5. Effect of overexpression of the LF and the SF on neural and neuronal markers. A-C: Expresssion of the neural marker N-CAM in uninjected embryos (uninj.), and in embryos injected with mRNA encoding either the LF or the SF as indicated above the photographs. D-F: Expression of the neuronal marker N-tubulin after the same set of injections. Arrowheads indicate ectopic activation of N-tubulin expression. G-I: Expression of the neuronal determination factor X-ngnr-1 after the same set of injections. J-L: Expression of the neuronal factor CRMP-4 after the same set of injections. M-O: Expression of the neuronal marker Xisl-1 after the same set of injections. |
