Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
isl1xenopus floor plate 

Too many results?Too few results?

Experiment details for isl1

Sulf1 influences the Shh morphogen gradient during the dorsal ventral patterning of the neural tube in Xenopus tropicalis.



Good quality Poor quality
Gene Clone Species Stages Anatomy
isl1 tropicalis NF stage 23 floor plate
isl1 tropicalis NF stage 40 floor plate

Display additional annotations [+]
  Fig. 2. Sulf1 is required for correct DV patterning in the vertebrate neural tube. Immunostaining for Nkx2.2 (A–D), Nkx6.1 (E–H), HB9 (I–L) and Isl1 (M–P) in control (A, B, E, F, I, J, M, N) and Sulf1 knockdown (C, D, G, H, K, L, O, P) X. tropicalis embryos at stages 23 (A–P) and stage 40 (A′–P′). At stage 23, the expression of Nkx2.2 is shifted ventrally in Sulf knockdown embryos (C, D; n=12, 75%) compared with controls (A, B; n=8, 100%). At stage 40, the expression of Nkx2.2 is also shifted ventrally in Sulf knockdown embryos (C′, D′; n=18, 67%) compared with controls (A′, B′; n=11, 100%). Sulf1 knockdown only leads to a small change in Nkx6.1 expression at stage 23 (G, H; n=8, 63%), compared to controls (E, F; n=8, 100%). At stage 40 the expression of Nkx6.1 is shifted ventrally (G′, H′; n=20, 70%), compared to controls (E,′ F′; n=12, 100%). HB9 staining similarly reveals differences between the stages, at stage 23, the expression of HB9 is reduced (K, L; n=12, 75%) compared with controls (I, J; n=7, 100%). Cells positive for HB9 in Sulf1 knockdown embryos increases at stage 40 (K′, L′; n=19, 69%). At stage 23, the expression of Isl1 reduced in the MN domain of Sulf knockdown embryos (O, P; n=8, 63%) compared with controls (M, N; n=12, 100%); whereas at stage 40 Isl1 is not reliably detectable in this domain in Sulf1 knockdowns (O′, P′; n=2, 100%) or in controls (M′, N′; n=5, 100%).
Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556