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Gene/CloneSpeciesStageAnatomy ItemExperimenter
isl1xenopus horizontal cell 

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Experiment details for isl1

Kha CX et al. (2018) Assay



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Gene Clone Species Stages Anatomy
isl1.L laevis NF stage 35 and 36 to NF stage 37 and 38 horizontal cell

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  Fig. 3. Regrown eye contains expected cell types. (A) Hematoxylin and eosin stained section of age-matched control (left panel) and regrown eyes at 5 dps (right panel). Regrown eye contains same eye structures compared to unoperated sibling control. (B-D) Identification of retinal cell types in eye regenerates. Dark pigmented tissue surrounding the retina is the retinal pigmented epithelium (RPE). (B) Eye sections stained with DAPI (DNA), anti-Rhodopsin antibody (rod photoreceptors), and anti-Calbindin-D-28K antibody (cone photoreceptors). (C) Eye sections stained with DAPI, anti-Isl1 antibody (identifies retinal ganglion cells, amacrine cells, bipolar cells, and horizontal cells). (D) Eye sections stained with DAPI, and anti-Glutamine Synthetase (Müller glia). (A-D) Up = dorsal, down = ventral. Scale bars: A-D = 50 μm.
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