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Fig. 6. Voltage-dependent potassium 4.2 (Kv4.2)–like subunits are
expressed in the Xenopus retina. Transverse sections through Xenopus
retinas at different developmental stages. A: Kv4.2-like immunoreactivity
is first detectable at stage 24 in the retina with labelling in
the basal lamina surrounding the eye primordium (arrowhead). B: At
stage 28, additional labelling is observed in radially oriented fibres
(arrowhead), processes along the vitreal surface of the retina (arrow),
and cell bodies. C: Higher magnification picture demonstrates staining
of processes along the vitreal surface (vs) in a stage 30 retina.
Arrows point to immunolabelling of the basal lamina. D: At stage
37/38, and earlier at stage 35/36, radial cell processes and a subset of
retinal ganglion cell and cells in the outer nerve layer (ONL) are
immunopositive for the Kv4.2-like protein. D, dorsal; Di, diencephalon;
Ep, eye primordium; IPL, inner plexiform layer; L, lens; RGCL,
retinal ganglion cell layer; V, ventral. Scale bar 200 m for A and
B and 100 m for C and D. |
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Fig. 7. Voltage-dependent potassium (Kv) subunits are differentially
expressed in developing Xenopus retinal ganglion cell (RGC)
growth cones. Stage 24 eye primordia were grown in culture for 24–48
hours and labelled with various Kv subunit antibodies. A: Phase
micrograph of the same RGC axons shown in B. Arrowhead points to
a filopodia. B: The Kv1.3 channel antibody labels the axon abundantly,
but not the body of the growth cone, nor the filopodia or
lamellipodia. C: The Kv1.5 antibody labels the RGC axon and the
entire growth cone, including the filopodia (arrowhead). D: The Kv4.2
antibody labels the RGC axon and growth cone in a punctate manner.
Scale bar 16 m for A–D. |
