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Figure 4. Immunohistochemistry of XAK-C and XAK-B. Sections of skin tissues were prepared from tadpoles at stage 56 (St. 56, A), 58 (B,C), and 61 (D), and adults (G) and subjected to immunohistochemistry for XAK-C (A,B,D,G) and XAK-B (C,E,H). Red and green color represent stains of XAK-C and XAK-B, respectively. F and I are the merged images of D and E, and G and H, respectively. There was no XAK-C signal at stage 56. Basal cells at stage 58 were XAK-C positive, but no cells were positive for XAK-B. Basal cells of the skin at stage 61 and of adults were stained with antiAK-C antisera. XAK-B signals were detected in differentiated cells seen on both sections. Immunohistochemistry experiments were performed four times, and similar staining patterns were obtained reproducibly. Scale bar = 20 μm in I (applies to A). |
