|
Fig. 5. Promoter activity of 5′-
flanking region of xak-c gene in an
F0 transgenic frog. A brightly
EGFP-fluorescent transgenic embryo
was developed to adulthood and
observed under a fluorescent
microscope anteriorly (A), dorsally
(B), and posteriorly (C). All images
were taken from the dorsal side.
Bars, 1 mm. Skin tissues were
removed from the back and
sectioned for immunostaining with
anti-XAK-C (D) and anti-GFP
antibodies (E). The sections were
also stained with Hoechst 33342
for nuclear staining. Bars, 50 μM.
bs, basal cell; c, cornified cells;
ct, connective tissue; gl, granular
gland; gr, granular cell. Dotted lines
indicate the basement membrane. |