|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
rgn
|
|
laevis
|
NF stage 35 and 36
|
pronephric nephrostome
,
pronephric kidney
|
pax8
|
|
xenopus
|
NF stage 22
|
mesoderm
,
intermediate mesoderm
,
pronephric mesenchyme
,
lateral
|
pax2
|
|
xenopus
|
NF stage 35 and 36
|
otic vesicle
,
spinal cord
,
pronephric nephrostome
,
pronephric duct
,
branchial arch
,
[+]
|
nphs1
|
|
xenopus
|
NF stage 35 and 36
|
pronephric mesenchyme
,
pronephric kidney
|
myod1
|
|
xenopus
|
NF stage 35 and 36
|
somite
,
branchial arch
|
|
|
Fig. 3. Effects of XPteg knockdown on pronephric mesoderm formation. Depletion of XPteg inhibits the expression of Lim1, Pax2, SMP30 and Pax8 that are related to pronephric tubules but not that of Nephrin and MyoD, a pronephric glomus and somatic mesoderm marker, respectively. These effects of MO were limited to the proximal tubules (white arrowheads) and rescued by coexpression of XPteg RNA. Eight-cell stage embryos were injected with β-galactosidase RNA as a lineage tracer along with MO (10 ng) and/or RNA into the presumptive pronephros region and later, stained with Red-Gal and subjected to in situ hybridization. One representative data are shown from three independent experiments. |