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lhx1xenopus   

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Experiment details for lhx1



XPteg (Xenopus proximal tubules-expressed gene) is essential for pronephric mesoderm specification and tubulogenesis.

Gene Clone Species Stages Anatomy
lhx1 xenopus NF stage 35 and 36 spinal cord , mesoderm , pronephric nephrostome , pronephric duct , pronephric mesenchyme , [+]

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  Fig. 3. Effects of XPteg knockdown on pronephric mesoderm formation. Depletion of XPteg inhibits the expression of Lim1, Pax2, SMP30 and Pax8 that are related to pronephric tubules but not that of Nephrin and MyoD, a pronephric glomus and somatic mesoderm marker, respectively. These effects of MO were limited to the proximal tubules (white arrowheads) and rescued by coexpression of XPteg RNA. Eight-cell stage embryos were injected with β-galactosidase RNA as a lineage tracer along with MO (10 ng) and/or RNA into the presumptive pronephros region and later, stained with Red-Gal and subjected to in situ hybridization. One representative data are shown from three independent experiments.