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Fig. 7. XPteg is induced directly by RA signaling. XPteg and Lim1 are induced by retinoic acid even in the presence of cycloheximide (CHX), a protein synthesis inhibitor. Whole embryos, which were treated with DMSO, ethanol, retinoic acid (10 μM) and/or CHX (10 μg/ml) for 2 h from stage 12.5, were in situ hybridized against XPteg or Lim1 at the indicated stages. In the case of RA + CHX (E, E′, J, J′ and J′), embryos were treated with CHX for 30 min prior to co-treatment of RA and CHX. DMSO and EtOH serve as negative controls for RA and CHX, respectively. White arrowheads in C′, E′ and red dots in H′, J′ denote enlarged ectopic pronephric tubules. |
