|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf4
|
|
laevis
|
NF stage 12
|
mesoderm
|
fgf8
|
|
laevis
|
NF stage 12
|
mesoderm
,
involuting marginal zone
,
upper blastopore lip
,
lower blastopore lip
|
adamts1
|
|
laevis
|
NF stage 8
to
NF stage 11
|
ectoderm
,
neuroectoderm outer layer
,
mesoderm
,
sensorial layer of neurectoderm
,
dorsal
,
[+]
|
adamts1
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
mesoderm
,
marginal zone
,
dorso-lateral marginal zone
|
adamts1
|
|
laevis
|
NF stage 26
|
fin
,
brain
,
hindbrain
,
hyoid crest
,
branchial crest
,
[+]
|
adamts1
|
|
laevis
|
NF stage 31
|
fin
,
olfactory placode
,
brain
,
hindbrain
,
head region
,
[+]
|
adamts1
|
|
laevis
|
NF stage 18
|
embryo
,
posterior
|
|
|
Fig. 2. Analysis of XADAMTS1 expression. (A) Northern blot analysis. Total RNA (10 μg) from different developmental stages was loaded in each lane. Bands of 18S rRNA stained with ethidium bromide were used for a loading control. (B) Whole-mount in situ hybridization for XADAMTS1 mRNA during blastula to neurula stages. (a, b) Late blastulae (stage 8.5) bisected vertically after staining for XADAMTS1 (a) and FGF8 (b). Magnified images of the enclosed regions are shown in the inset. (c) Comparison of expression domains between XADAMTS1 and Xlim-1 at the gastrula stage (stage 10.5). Embryos were bisected into left and right halves and subjected to whole-mount in situ hybridization for XADAMTS1 (left panel) and Xlim-1 (right panel), respectively. Dorsal is to the right (XADAMTS1) or the left (Xlim-1). (d) Vegetal view of a mid-gastrula embryo (stage 11.5) cleared with BB/BA. (e–g) Late gastrulae (stage 12) bisected sagittally after staining for XADAMTS1 (e), FGF4 (f), and FGF8 (g). Dorsal is upwards; anterior is to the left. (h) Dorsal view of neurula (stage 18). Anterior is upwards. (C) Expression patterns of XADAMTS1 at tailbud stages. (a) Early tailbud stage embryo (stage 26). Transverse (b–d) and horizontal (e) sections of stained tailbud stage embryos (stage 26). (f) Late tailbud stage embryo (stage 31) cleared with BB/BA. (g) Transverse section of a stained late tailbud stage embryo (stage 31). Dorsal is upwards (a–d, f, g). Anterior is to the left (a, e, f). ba, branchial arch; d, dermatome; e, eye; h, heart anlage; n, notochord; nt, neural tube; ov, otic vesicle; pn, pronephros. Arrowheads indicate the dorsal blastopore. |