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FIG. 2. XPax-8 and Xlim-1 expression in the presumptive embryonic kidney. Expression of Pax-8 as revealed by whole-mount in situ hybridization of Xenopus embryos of various developmental stages (samples on the left). Xlim-1 (Taira et al., 1994a) expression at similar stages is illustrated on the right. The two distinct
patterns of pronephric expression converge until they are indistinguishable by stage 25. A, stage14, XPax-8. B, stage 14, Xlim-1. C,
stage 20, XPax-8. D, stage 20, Xlim-1. E, stage 24, XPax-8. F, stage 23, Xlim-1. G, double staining of stage 16 embryo with XPax-8 in dark brown and Xlim-1 in pink/red. Computer-generated false
coloring was used to enhance the difference between the two
immunohistochemical substrates. H, graphical interpretation of data shown in G. Green represents XPax-8 only expression domains in the presumptive otic vesicle and possibly in the posterior pronephric region. Red represents the Xlim-1 only domain in the ventral mesoderm belt. Blue represents the area of overlap. I, graphical representation of data in E and F; both XPax-8 and Xlim-1 are expressed in overlapping domains throughout the presumptive pronephros (blue). ot, otic vesicle; pn, pronephros. Anterior is to the
left, and dorsal up, in all samples. |
