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Fig. 2. Lim1 in the dorsal blas- topore lip. In situ hybridization against Xlim1 mRNA is shown in (A); the remaining embryos were immunostained against Lim1 protein. (A,B) Early gastrulae of X. laevis. (A) Xlim1 mRNA expres- sion. (B) The dorsal blastopore lip in sagittal view reveals the internal Lim1 protein signal. (C,D) Gastrulae of E. randi. (C) Surface Lim1 protein signal in the dorsal blastopore lip. (D) The dorsal blas- topore lip in sagittal view. The dark line in the middle of the image is due to unspecific stain- ing of the blastocoel floor. (E,F) Early gastrulae of C. machalilla. (E) The surface Lim1-signal is visible in the dorsal blastopore lip, and in a belt around the blastopore. (F) The dorsal blastopore lip in sagittal view reveals the internal signal. (G,H) Mid gastrulae of G. riobambae. (G) Lim1 signal at st 12 occurs in a belt around the blastopore with a strong dorsal expression. (H) The blastopore of a st 12 embryo in sagittal view. A higher density of Lim1-positive nuclei were detected in the dorsal blastopore lip in comparison with the ventral lip. Abbreviations: dl, dorsal lip; vl, ventral lip; yp, yolk plug. Scale bars: 200 μm (A,E,G,H); 100 μm (B,C,D,F). |
