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Fig. 5. Effect of Xbf2 mRNA on mesodermal development. Embryos were injected in one cell at the 2-cell stage in the equatorial region with 4 ng of Xbf2 mRNA and 0.3 ng of lacZ mRNA and cultured until stage 10.5-11.5, when the expression of different mesodermal markers were analyzed. Arrowheads point to the injected sites. Neither gsc (A) nor Xlim1 (B) expression were affected by Xfb2 overexpression (100%, n = 18 and 13, respectively). (C) XMyoD expression was strengthened in the lateral mesoderm in the injected side (60%, n = 34). (D) Ventral or lateral Xbf2 injections did not affect Xvent2 expression (94%, n = 48). In contrast, overexpression of Xbf2 strongly inhibited the expression ofXwnt8 (E; 95%, n = 45) and Xvent1 (F; 92% n = 23) and weakly that of BMP-4 (G; 80% n = 15) and Xmsx1 (H; 58%, n = 12) at the injection site. |
